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Генома белок-белковых Взаимодействие Скрининг белком-фрагментом комплементации анализа (РСА) в живых клетках
JoVE Journal
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Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay (PCA) in Living Cells
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Генома белок-белковых Взаимодействие Скрининг белком-фрагментом комплементации анализа (РСА) в живых клетках

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08:38 min

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March 03, 2015

DOI:

10.3791/52255-v

DOI:
10.3791/52255-v

•

08:38 min
March 03, 2015

•

10 Views
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1Département de Biologie, Institut de biologie intégrative et des systémes & PROTEO,Université Laval

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Proteins interact with each other and these interactions determine in a large part their functions. Protein interaction partners can be identified at high-throughput in vivo using a yeast fitness assay based on the dihydrofolate reductase protein-fragment complementation assay (DHFR-PCA).

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Protein-protein InteractionProtein-fragment Complementation AssayPCADHFRMethotrexateHigh-throughputGenome-wideLiving CellsYeast

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Rochette, S., Diss, G., Filteau, M., Leducq, J., Dubé, A. K., Landry, C. R. Genome-wide Protein-protein Interaction Screening by Protein-fragment Complementation Assay (PCA) in Living Cells. J. Vis. Exp. (97), e52255, doi:10.3791/52255 (2015).

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