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Imagerie 3-D et de l'analyse des neurones infecté<em> In Vivo</em> Avec<em> Toxoplasma gondii</em
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JoVE Journal Nörobilim
3-D Imaging and Analysis of Neurons Infected In Vivo with Toxoplasma gondii
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Imagerie 3-D et de l'analyse des neurones infecté<em> In Vivo</em> Avec<em> Toxoplasma gondii</em

11,346 Views

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06:33 min

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December 09, 2014

DOI:

10.3791/52237-v

DOI:
10.3791/52237-v

•

06:33 min
December 09, 2014

•

4 Views
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1Bio5 Institute,University of Arizona, 2Department of Neurology,University of Arizona, 3Department of Immunobiology,University of Arizona

Özet

Automatically generated

En utilisant ce protocole, nous avons pu coupes de cerveau l'image d'épaisseur de 160 um à partir de souris infectées par le parasite Toxoplasma gondii, qui permet la visualisation et l'analyse de la relation spatiale entre le parasite de enkyster et le neurone infecté.

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3-D ImagingNeuronsToxoplasma GondiiIntracellular ParasiteBrain InfectionCentral Nervous System (CNS)Neuron InfectionPersistent InfectionThree-dimensional VisualizationImmunostainingElectron MicroscopySerial Sectioning

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Cite this Article

Cabral, C. M., Koshy, A. A. 3-D Imaging and Analysis of Neurons Infected In Vivo with Toxoplasma gondii. J. Vis. Exp. (94), e52237, doi:10.3791/52237 (2014).

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