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2D電気泳動によるマクロファージのプロテオームプロファイリング
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November 04, 2014
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Macrophages are the key cells involved in host pathogenicity. Macrophages display phenotypic and functional diversity that can be analysed and detected by proteomic analysis. This article describes how to perform 2D electrophoresis of primary cultures of human macrophages differentiated into M1 or M2 phenotype.
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Bouvet, M., Turkieh, A., Acosta-Martin, A. E., Chwastyniak, M., Beseme, O., Amouyel, P., Pinet, F. Proteomic Profiling of Macrophages by 2D Electrophoresis. J. Vis. Exp. (93), e52219, doi:10.3791/52219 (2014).
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