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Isolement de cellules primaires murins cerveau endothéliales microvasculaires
JoVE Journal
Nörobilim
This content is Free Access.
JoVE Journal Nörobilim
Isolation of Primary Murine Brain Microvascular Endothelial Cells

Isolement de cellules primaires murins cerveau endothéliales microvasculaires

DOI:

08:14 min

November 14, 2014

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Bölümler

  • 00:05Başlık
  • 01:46Isolation of Murine Brain Microvascular Endothelial Cells (BMEC)
  • 05:41Results: Morphological and Functional Properties of Murine BMECs
  • 07:21Conclusion

Özet

Otomatik Çeviri

Brain microvascular endothelial cells (BMEC) are interconnected by specific junctional proteins forming a highly regulated barrier separating blood and the central nervous system (CNS), the so-called blood-brain-barrier (BBB). The isolation of primary murine brain microvascular endothelial cells, as discussed in this protocol, enables detailed in vitro studies of the BBB.

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