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サブクローニングプラス挿入（SPI） - ジーンターゲティングベクターの迅速な構築のための新しい遺伝子改変方法
JoVE Journal
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JoVE Journal Biyoloji
Subcloning Plus Insertion (SPI) – A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors
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サブクローニングプラス挿入（SPI） - ジーンターゲティングベクターの迅速な構築のための新しい遺伝子改変方法

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09:02 min

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January 08, 2015

DOI:

10.3791/52155-v

DOI:
10.3791/52155-v

•

09:02 min
January 08, 2015

•

1 Views
, , , ,
1Department of Biochemistry,University of Leicester, 2Center for Fisheries, Environment and Aquaculture Sciences, 3ES Cell Facility, Centre for Core Biotechnology Services,University of Leicester

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Gene targeting methodologies can be used to generate transgenic mice with knockout, knock-in and tagged alleles. Here, we describe an improved method of recombineering in E. coli, that we term ‘subcloning plus insertion’, which can be used to generate custom gene targeting vectors rapidly.

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Reddy, T. R., Kelsall, E. J., Fevat, L. M., Munson, S. E., Cowley, S. M. Subcloning Plus Insertion (SPI) - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors. J. Vis. Exp. (95), e52155, doi:10.3791/52155 (2015).

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