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亚克隆加插入（SPI） - 一种新型重组工程方法基因打靶载体的快速构建
Bölümler
- 00:05Başlık
- 01:04Multiplex Recombineering Oligos
- 02:00BAC Clone Transformation
- 03:56Insertion Cassettes and Subcloning Plasmids
- 05:03Subcloning Plus Insertion
- 07:07Analysis of Recombinants
- 07:48Results: Subcloning Plus Insertion (SPI)
- 08:39Conclusion
Gene targeting methodologies can be used to generate transgenic mice with knockout, knock-in and tagged alleles. Here, we describe an improved method of recombineering in E. coli, that we term ‘subcloning plus insertion’, which can be used to generate custom gene targeting vectors rapidly.
