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استنساخ فرعي زائد الإدراج (SPI) - رواية الطريقة Recombineering عن البناء السريع للجين استهداف ناقلات
Bölümler
- 00:05Başlık
- 01:04Multiplex Recombineering Oligos
- 02:00BAC Clone Transformation
- 03:56Insertion Cassettes and Subcloning Plasmids
- 05:03Subcloning Plus Insertion
- 07:07Analysis of Recombinants
- 07:48Results: Subcloning Plus Insertion (SPI)
- 08:39Conclusion
Gene targeting methodologies can be used to generate transgenic mice with knockout, knock-in and tagged alleles. Here, we describe an improved method of recombineering in E. coli, that we term ‘subcloning plus insertion’, which can be used to generate custom gene targeting vectors rapidly.
