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Региоселективный Biolistic Ориентация в Органотипической мозга Ломтики Использование модифицированного гена Gun
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JoVE Journal Nörobilim
Regioselective Biolistic Targeting in Organotypic Brain Slices Using a Modified Gene Gun
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Региоселективный Biolistic Ориентация в Органотипической мозга Ломтики Использование модифицированного гена Gun

16,028 Views

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06:40 min

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October 24, 2014

DOI:

10.3791/52148-v

DOI:
10.3791/52148-v

•

06:40 min
October 24, 2014

•

6 Views
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1Leslie Dan Faculty of Pharmacy,University of Toronto, 2MRC-Laboratory of Molecular Biology, Cambridge, UK

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Recent improvements in organotypic brain slice preparations have permitted their exploitation for biotechnological applications. Organotypic slices maintain local structural characteristics of in vivo biology, including functional synaptic connections. Here we present a regioselective biolistic delivery method to label and genetically manipulate these slices.

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Arsenault, J., Nagy, A., Henderson, J. T., O'Brien, J. A. Regioselective Biolistic Targeting in Organotypic Brain Slices Using a Modified Gene Gun. J. Vis. Exp. (92), e52148, doi:10.3791/52148 (2014).

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