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位置選択的微粒子銃は、改変された遺伝子銃を使用した器官型脳切片におけるターゲティング
JoVE Journal
Nörobilim
This content is Free Access.
JoVE Journal Nörobilim
Regioselective Biolistic Targeting in Organotypic Brain Slices Using a Modified Gene Gun

位置選択的微粒子銃は、改変された遺伝子銃を使用した器官型脳切片におけるターゲティング

DOI:

06:40 min

October 24, 2014

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Bölümler

  • 00:05Başlık
  • 01:21Preparation of Organotypic Slices
  • 02:20Preparation of DNA-coated Micro & Nano-projectiles
  • 03:38Biolistic Transfection on Organotypic Slices
  • 04:29Fixing and Visualization of Brain Slices
  • 05:24Results: Biolistic Delivery of Fluorescent Protein Encoded DNA into Organotypic Slices
  • 06:12Conclusion

Özet

Otomatik Çeviri

Recent improvements in organotypic brain slice preparations have permitted their exploitation for biotechnological applications. Organotypic slices maintain local structural characteristics of in vivo biology, including functional synaptic connections. Here we present a regioselective biolistic delivery method to label and genetically manipulate these slices.

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