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Derivación de células T<em> In Vitro</em> De células madre embrionarias de ratón
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JoVE Journal İmmünoloji ve Enfeksiyon
Derivation of T Cells In Vitro from Mouse Embryonic Stem Cells
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Derivación de células T<em> In Vitro</em> De células madre embrionarias de ratón

10,801 Views

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08:27 min

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October 14, 2014

DOI:

10.3791/52119-v

DOI:
10.3791/52119-v

•

08:27 min
October 14, 2014

•

4 Views
, , , , ,
1Department of Biological Sciences, Hunter College and Graduate Center,City University of New York, 2Sunnybrook Research Institute, Department of Immunology,University of Toronto

Özet

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Mouse embryonic stem cells can be differentiated to T cells in vitro using the OP9-DL1 co-culture system. Success in this procedure requires careful attention to reagent/cell maintenance, and key technique sensitive steps. Here we discuss these critical parameters and provide a detailed protocol to encourage adoption of this technology.

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Mouse Embryonic Stem CellsOP9/OP9-DL1 Co-culture SystemHematopoietic ProgenitorsHematopoiesisT Lymphocyte DifferentiationIn Vitro DifferentiationCell Culture MaintenanceProtocolTechnique Sensitive Steps

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Kučerová-Levisohn, M., Lovett, J., Lahiji, A., Holmes, R., Zúñiga-Pflücker, J. C., Ortiz, B. D. Derivation of T Cells In Vitro from Mouse Embryonic Stem Cells. J. Vis. Exp. (92), e52119, doi:10.3791/52119 (2014).

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