/
/
Groß Reinigung von Schwein oder Rind Photorezeptoraußensegmente für Phagozytose Assays auf Netzhautpigmentepithelzellen
Bu içeriği görüntülemek için JoVE aboneliği gereklidir. Oturum açın veya ücretsiz deneme sürümünü başlatın.
Groß Reinigung von Schwein oder Rind Photorezeptoraußensegmente für Phagozytose Assays auf Netzhautpigmentepithelzellen
Bölümler
- 00:05Başlık
- 02:01Set-up, Solution and Gradient Preparation
- 03:20Tissue Collection and Retina Homogenization
- 04:54Photoreceptor Outer Segment Fragment Isolation
- 05:58POS Aliquoting Without and With Labeling
- 07:48Results: Large-scale Purification of Photoreceptor Outer Segments
- 09:28Conclusion
This article describes the protocol for the purification of photoreceptor outer segment fragments (POS) via ultracentrifugation from porcine/bovine retinae using homogenization and sucrose gradient centrifugation. This protocol allows the preparation of large stocks of POS aliquots, labeled or unlabeled, that can then be stored at -80 °C.
