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通过胞内细菌，研究内溶酶体系统的改造荧光纳米粒子中的应用
JoVE Journal
İmmünoloji ve Enfeksiyon
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JoVE Journal İmmünoloji ve Enfeksiyon
Application of Fluorescent Nanoparticles to Study Remodeling of the Endo-lysosomal System by Intracellular Bacteria
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通过胞内细菌，研究内溶酶体系统的改造荧光纳米粒子中的应用

DOI:
10.3791/52058-v

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11:38 min

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January 02, 2015

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, ,
1Abteilung Mikrobiologie, Fachbereich Biologie/Chemie,Universität Osnabrück

Bölümler

  • 00:05Başlık
  • 01:26Synthesis of 10-nm Gold Nanoparticles (Gold NPs)
  • 02:28Coating of Gold NPs with BSA and Labeling with Rhodamine N-hydroxysuccinimidyl Ester (NHS)
  • 04:30Culture of HeLa Cells and Salmonella Bacteria
  • 05:40Infection of HeLa Cells by Salmonella and Gold-BSA-Rhodamine NPs Pulse Chase-labeling
  • 07:04Imaging and Image Analysis
  • 09:18Results: Salmonella Infection Rearranges the Endolysosomal System in HeLa Cells
  • 10:58Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

本文介绍了合成和纳米粒子的荧光标记（NPS）的方法。所述纳米颗粒是在脉冲追踪实验施加标记的真核细胞的内溶酶体系统。内溶酶体系统的细胞内病原体沙门氏菌的活动操纵其次是活细胞成像和量化。

Etiketler

Fluorescent NanoparticlesEndo-lysosomal SystemIntracellular BacteriaSalmonella EntericaHeLa CellsImaris SoftwareConfocal MicroscopyQuantitative AnalysisOrganelle LabelingHost-pathogen Interactions

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