Bu içeriği görüntülemek için JoVE aboneliği gereklidir. Oturum açın veya ücretsiz deneme sürümünü başlatın.
主要組織適合遺伝子複合体クラスII Dextramersを用いて脳および心臓において自己反応性CD4 T細胞のその場検出に
Bölümler
- 00:05Başlık
- 01:49In Situ Staining of Cerebral Sections with MHC Class II (IAs)/PLP 139-151 Dextramers
- 06:28Analysis of Dextramer+ (dext+) CD4+ T Cells by Laser Scanning Confocal Microscopy (LSCM)
- 10:50Results: In Situ Detection of Antigen-specific Autoreactive CD4 T Cells by Direct Staining with MHC Class II Dextramers
- 12:29Conclusion
Etiketler
MHC Class II DextramersAutoreactive CD4 T CellsExperimental Autoimmune Encephalomyelitis (EAE)Experimental Autoimmune Myocarditis (EAM)Laser Scanning Confocal Microscope (LSCM)Myelin Proteolipid Protein (PLP) 139-151Cardiac Myosin Heavy Chain-α (Myhc) 334-352Theiler’s Murine Encephalomyelitis Virus (TMEV) 70-86Bovine Ribonuclease (RNase) 43-56In Situ DetectionQuantitative Analysis
