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설치류 부고환 정자의 포스 포 분석
JoVE Journal
Biyoloji
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JoVE Journal Biyoloji
Phosphopeptide Analysis of Rodent Epididymal Spermatozoa
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설치류 부고환 정자의 포스 포 분석

12,687 Views

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09:30 min

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December 30, 2014

DOI:

10.3791/51546-v

DOI:
10.3791/51546-v

•

09:30 min
December 30, 2014

•

5 Views
, , ,
1School of Environmental and Life Science,University of Newcastle, 2Drug Delivery, Disposition and Dynamics, Monash Institute of Pharmaceutical Sciences,Monash University

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Proteomic analysis of any cell type is highly dependent on both purity and pre-fractionation of the starting material in order to de-complexify the sample prior to liquid chromatography mass spectrometry (MS). By using back-flushing techniques, pure spermatozoa can be obtained from rodents. Following digestion, phosphopeptides can be enriched using TiO2.

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Baker, M. A., Hetherington, L., Weinberg, A., Velkov, T. Phosphopeptide Analysis of Rodent Epididymal Spermatozoa. J. Vis. Exp. (94), e51546, doi:10.3791/51546 (2014).

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