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Two-Photon<em> In vivo</em> Imagem de espinhas dendríticas no Rato Cortex Usando um desbaste-crânio Preparação
JoVE Journal
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JoVE Journal Nörobilim
Two-Photon in vivo Imaging of Dendritic Spines in the Mouse Cortex Using a Thinned-skull Preparation

Two-Photon<em> In vivo</em> Imagem de espinhas dendríticas no Rato Cortex Usando um desbaste-crânio Preparação

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09:53 min

May 12, 2014

DOI:

09:53 min
May 12, 2014

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Özet

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Time-lapse imaging in the living animal provides valuable information on structural reorganization in the intact brain. Here, we introduce a thinned-skull preparation that allows transcranial imaging of fluorescently labeled synaptic structures in the living mouse cortex by two-photon microscopy.

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