'Dergi'
/
Biyoloji
/
Polysome Fractionation and Analysis of Mammalian Translatomes on a Genome-wide Scale
JoVE Journal
Biyoloji
This content is Free Access.
JoVE Journal Biyoloji
Polysome Fractionation and Analysis of Mammalian Translatomes on a Genome-wide Scale

Polysome Fractionation and Analysis of Mammalian Translatomes on a Genome-wide Scale

DOI:
10.3791/51455-v

•

10:56 min

•

May 17, 2014

•

, , , , , ,
1Lady Davis Institute and Department of Oncology,McGill University, 2Department of Oncology-Pathology,Karolinska Institutet, 3Goodman Cancer Centre and Department of Biochemistry,McGill University

'Bölümler'

  • 00:05'Başlık'
  • 01:54Preparation of Sucrose Gradient
  • 03:38Isolation and Sedimentation of Polysomes
  • 06:59Polysome Fractionation and RNA Extraction
  • 09:22Results: Analytics of Translational Activity in MCF7 Cells Using Polysome Fractionation
  • 10:24Conclusion

Özet

'Otomatik Çeviri'

'Otomatik Çeviri'

Ribosomes play a central role in protein synthesis. Polyribosome (polysome) fractionation by sucrose density gradient centrifugation allows direct determination of translation efficiencies of individual mRNAs on a genome-wide scale. In addition, this method can be used for biochemical analysis of ribosome- and polysome-associated factors such as chaperones and signaling molecules.

'Etiketler'

MRNA TranslationTranslatomeRibosomePolysomeSucrose Density Gradient CentrifugationAnota AlgorithmGenome-wide AnalysisProtein FoldingPost-translational Modifications

Article

Embed

'ÇALMA LİSTESİNE EKLE'

Usage Statistics

'İlgili Videolar'

Intranuclear Microinjection of DNA into Dissociated Adult Mammalian Neurons

In situ Subcellular Fractionation of Adherent and Non-adherent Mammalian Cells

Genome-wide Analysis of Aminoacylation (Charging) Levels of tRNA Using Microarrays

Genome-wide Analysis using ChIP to Identify Isoform-specific Gene Targets

Mutagenesis and Functional Analysis of Ion Channels Heterologously Expressed in Mammalian Cells

Scale-Up of Mammalian Cell Culture using a New Multilayered Flask

Analysis of Cell Cycle Position in Mammalian Cells

A Novel Bayesian Change-point Algorithm for Genome-wide Analysis of Diverse ChIPseq Data Types

Genome-wide Gene Deletions in Streptococcus sanguinis by High Throughput PCR

Analysis of Translation Initiation During Stress Conditions by Polysome Profiling

Read Article