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核苷三磷酸 - 从合成到生化特征

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Nucleoside Triphosphates – From Synthesis to Biochemical Characterization

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核苷三磷酸 - 从合成到生化特征

DOI:

10.3791/51385-v

•

15:22 min

•

April 03, 2014

•

Marcel Hollenstein, Christine Catherine Smith, Michael Räz

¹Department of Chemistry and Biochemistry,University of Bern

Bölümler

00:05Başlık
02:30Synthesis of the Modified Nucleoside Triphosphates
05:56HPLC Purification of the Modified Nucleoside Triphosphates
07:40Primer Extension Reactions and TdT Polymerizations
11:05Results: Biochemical Characterization of Nucleoside Triphosphates
14:02Conclusion

Özet

Otomatik Çeviri

本文所描述的协议旨在解释和删节的复杂路线导致修饰的三磷酸核苷的方法的许多障碍。因此，该协议有利于激活这些构建模块两者的合成及其可用性的实际应用。

Etiketler

Nucleoside Triphosphates Phosphoramidite Precursors Solid-phase Synthesis Modified Nucleic Acids Functional Tagging Ribozymes DNAzymes Phosphorous(III)-based Reagents Primer Extension Reactions TdT Tailing Polymerization Chemical Biology

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