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Laser-induced Breakdown Spectroscopy: A New Approach for Nanoparticle’s Mapping and Quantification in Organ Tissue
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JoVE Journal Mühendislik
Laser-induced Breakdown Spectroscopy: A New Approach for Nanoparticle’s Mapping and Quantification in Organ Tissue

Laser-induced Breakdown Spectroscopy: A New Approach for Nanoparticle’s Mapping and Quantification in Organ Tissue

DOI:
10.3791/51353-v

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10:17 min

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June 18, 2014

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, , , , , , ,
1ILM-FENNEC UMR 5306,CNRS – Université Lyon 1, 2ILM-PUBLI UMR 5306,CNRS – Université Lyon 1, 3ILM-SOPRANO UMR 5306,CNRS – Université Lyon 1

Bölümler

  • 00:05Başlık
  • 02:45Biological Sample Preparation and Calibration Sample Preparation
  • 04:03The Laser Induced Breakdown Spectroscopy (LIBS) Setup
  • 05:56Mapping Measurement and Calibration Measurement
  • 07:16Results: LIBS Spectra and Chemical Maps of Mouse Tissue
  • 09:02Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

Laser-induced breakdown spectroscopy performed on thin organ and tumor tissue successfully detected natural elements and artificially injected gadolinium (Gd), issued from Gd-based nanoparticles. Images of chemical elements reached a resolution of 100 μm and quantitative sub-mM sensitivity. The compatibility of the setup with standard optical microscopy emphasizes its potential to provide multiple images of a same biological tissue.

Etiketler

Laser-induced Breakdown SpectroscopyLIBSNanoparticlesMappingQuantificationElemental AnalysisBiological SamplesKidneysTumorInorganic ElementsSodiumCalciumCopperMagnesiumPhosphorusIronSiliconGadoliniumGadolinium-based NanoparticlesQuantitative Chemical ImagingSub-millimolar Sensitivity

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