Bu içeriği görüntülemek için JoVE aboneliği gereklidir. Oturum açın veya ücretsiz deneme sürümünü başlatın.
Isolation and Quantification of Botulinum Neurotoxin From Complex Matrices Using the BoTest Matrix Assays
Bölümler
- 00:05Başlık
- 01:40Preparation of the Standard Curve
- 03:33Prepare Solid Food Unknown Samples
- 04:56Plate Setup and BoNT/A Pull Down
- 06:11Assay Initiation, Incubation, and Data Collection
- 07:14Results: BoTest Matrix Assay
- 11:43Conclusion
The BoTest Matrix botulinum neurotoxin (BoNT) detection assays rapidly purify and quantify BoNT from a range of sample matrices. Here, we present a protocol for the detection and quantification of BoNT from both solid and liquid matrices and demonstrate the assay with BOTOX, tomatoes, and milk.
