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细胞表面和内蛋白质的现场培养神经细胞的抗体后喂养差异化标记
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JoVE Journal Nörobilim
Differential Labeling of Cell-surface and Internalized Proteins after Antibody Feeding of Live Cultured Neurons
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细胞表面和内蛋白质的现场培养神经细胞的抗体后喂养差异化标记

DOI:
10.3791/51139-v

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11:56 min

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February 12, 2014

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, , , ,
1Department of Anatomy & Neuroscience,The University of Melbourne, 2Department of Neurobiology,Duke University Medical Center, 3Florey Institute of Neuroscience & Mental Health,The University of Melbourne

Bölümler

  • 00:05Başlık
  • 01:28Preparation of Dissociated Primary Embryonic Neuron Culture
  • 04:56Antibody Incubation with Live Neurons
  • 10:26Representative Imaging Results
  • 11:33Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

我们描述了一种方法，以使用特定的多克隆抗体对表位的细胞外活神经元的表面上的标记蛋白。蛋白结合的抗体在细胞表面上，并随后通过胞吞作用内在化可以从蛋白质上残留进行区分，或在培养过程中贩卖到，该表面。

Etiketler

Differential LabelingCell-surface ProteinsInternalized ProteinsAntibody FeedingLive Cultured NeuronsTransmembrane ReceptorProtein TraffickingEndocytosisFluorescent Secondary AntibodySubcellular Localization

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