/
/
Novel Whole-tissue Quantitative Assay of Nitric Oxide Levels in Drosophila Neuroinflammatory Response
This content is Free Access.
Novel Whole-tissue Quantitative Assay of Nitric Oxide Levels in Drosophila Neuroinflammatory Response
7,802 Views
•
07:49 min
•
December 04, 2013
DOI:
Özet
Automatically generated
Levels of the inflammatory cell-signaling molecule nitric oxide (NO) are commonly assayed using Griess reagent. In this protocol, we have created a modified Griess assay utilizing live Drosophila brain tissue in order to detect the secretion of NO in a simple, quantifiable and highly repeatable method.
Read Article
Cite this Article
Ajjuri, R. R., O'Donnell, J. M. Novel Whole-tissue Quantitative Assay of Nitric Oxide Levels in Drosophila Neuroinflammatory Response. J. Vis. Exp. (82), e50892, doi:10.3791/50892 (2013).
Copy