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CA同时测量人的心房肌细胞分离<sup> +</sup>瞬变和膜电流
JoVE Journal
Biyoloji
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JoVE Journal Biyoloji
Isolation of Human Atrial Myocytes for Simultaneous Measurements of Ca2+ Transients and Membrane Currents
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CA同时测量人的心房肌细胞分离<sup> +</sup>瞬变和膜电流

DOI:
10.3791/50235-v

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10:53 min

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July 03, 2013

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, ,
1Institute of Pharmacology,University of Duisburg-Essen , 2Division of Experimental Cardiology,University of Heidelberg

Bölümler

  • 00:05Başlık
  • 01:26Cleaning the Tissue
  • 03:11Enzymatic Digestions and Precipitation
  • 06:33Loading of Myocytes with Fluo-3AM
  • 07:53Representative Cardiomyocytes and Calcium Transient Recordings
  • 10:24Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

我们描述了人类心房肌细胞可用于细胞内Ca隔离<sup> +</sup>组合测量膜片钳电生理研究。

Etiketler

Human Atrial MyocytesPatch-clampCa2+ TransientsMembrane CurrentsAtrial FibrillationCell IsolationCollagenaseProteaseCa2+ Buffering

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