This content is Free Access.
De procéder au lavage vaginal, coloration au cristal violet et l'évaluation cytologique vaginal pour Souris cycle œstral Staging identification
106,992 Views
•
09:01 min
•
September 15, 2012
DOI:
1Department of Biochemistry, Microbiology and Immunology,Neural Regeneration Laboratory and Ottawa Institute of Systems Biology, 2Department of Cellular and Molecular Medicine,University of Ottawa , 3CIHR Program in Neurodegenerative Lipidomics,University of Ottawa , 4Carleton Immersive Media Studio,Azrieli School of Architecture and Urbanism
Özet
Automatically generated
Ici, nous décrivons comment identifier le stade de la reproduction murin (proestrus, oestrus, metestrus ou diestrus) par simple, non invasive collecte et l'évaluation cytologique des échantillons frottis vaginaux. En outre, nous décrivons comment la cytologie vaginale reflète les taux circulants hormonaux qui sous-tendent la transition dans le cycle de reproduction murin.
Explore More Videos
Vaginal LavageCrystal Violet StainingVaginal Cytological EvaluationMouse Estrous Cycle StagingReproductive StatusMouse Models Of DiseaseHormonal RegulationPathological ChallengeProestrusEstrusMetestrusDiestrusOvarian Steroids17-beta-estradiolProgesteroneLuteinizing HormoneFollicle Stimulating HormoneProlactinCell TypologyNucleated Epithelial CellsCornified Squamous Epithelial CellsLeukocytesVaginal SmearsInvasivenessNon-invasive Protocol
Read Article
Cite this Article
McLean, A. C., Valenzuela, N., Fai, S., Bennett, S. A. Performing Vaginal Lavage, Crystal Violet Staining, and Vaginal Cytological Evaluation for Mouse Estrous Cycle Staging Identification. J. Vis. Exp. (67), e4389, doi:10.3791/4389 (2012).
Copy