This content is Free Access.
Human Neuroendocrine Tumor Cell Lines as a Three-Dimensional Model for the Study of Human Neuroendocrine Tumor Therapy
Bölümler
- 00:05Başlık
- 02:05Preparation of 1% Agarose-coated 24-well Plates
- 03:08Preparation of Single Cell Suspensions
- 04:17Spheroid Culture
- 05:31Drug Treatment and Monitoring of 3D Spheroid Cultures
- 07:22HistoGel-embedding of the NET 3D Spheroids
- 09:06Results: Culture of NET 3D Spheroids and Their Response to Drug Treatment
- 11:29Conclusion
We present a simple agarose overlay platform to grow 3D multicellular spheroids using neuroendocrine cancer cell lines. This method provides a very convenient way to examine the effect of therapeutic drugs on the neuroendocrine tumor cells. It could also help us establish human neuroendocrine tumor spheroids for cancer therapy.
Etiketler
Neuroendocrine TumorHuman Cell LinesThree-dimensional ModelStudyTherapyIncidenceMalignanciesSystemic TherapyBiyolojiTherapeutic TargetsNonresectableMetastatic Neuroendocrine Tumors3D Cell CultureDrug ScreeningMicroenvironmentMulticellular SpheroidsIn Vitro ModelAgarose-coated PlatePhysiological ConditionsDrug Treatments
