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細胞侵襲DISC1タンパク質種の生成、精製とキャラクタリゼーション
JoVE Journal
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JoVE Journal Biyoloji
Generation, Purification, and Characterization of Cell-invasive DISC1 Protein Species
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細胞侵襲DISC1タンパク質種の生成、精製とキャラクタリゼーション

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14:33 min

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August 30, 2012

DOI:

10.3791/4132-v

DOI:
10.3791/4132-v

•

14:33 min
August 30, 2012

•

1 Views
, , , ,
1Department of Neuropathology,Medical School Düsseldorf, Germany, 2Center of Behavioral Neuroscience,University of Düsseldorf

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細胞培養液からの細胞内、細胞質の全長DISC1タンパク質のアグレソームのとで標識し、多量換えDISC1タンパク質断片の生成、精製および細胞浸潤<em> E大腸菌の</em>記載されています。細胞の浸潤は、細胞培養とニューロンの受容細胞のために示されて<em生体内で></em> stereotactical脳接種後。

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Bader, V., Ottis, P., Pum, M., Huston, J. P., Korth, C. Generation, Purification, and Characterization of Cell-invasive DISC1 Protein Species. J. Vis. Exp. (66), e4132, doi:10.3791/4132 (2012).

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