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Werkwijze voor het isoleren en identificeren van mRNAs, microRNA en eiwitcomponenten van ribonucleoproteïne complexen uit celextracten met RIP-Chip
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September 29, 2012
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Een stap voor stap-protocol om het isoleren en identificeren van RNA bijbehorende complexen door middel van RIP-Chip.
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IsolationIdentificationMRNAsMicroRNAsProtein ComponentsRibonucleoprotein ComplexesRIP-ChipHigh-throughput SequencingMicroarray AnalysisGlobal Gene Expression AnalysisPost-transcriptional Gene RegulationRNA Binding Proteins (RBP)MRNA LocalizationStabilityTranslationRibonucleoprotein (RNP) ComplexDe Novo MRNA TargetsCellular ExtractsProtein OutputRNP Immunoprecipitation-microarray (RIP-Chip)Experimental ConditionsPost-transcriptional Gene Regulation MechanismsRibonucleoprotein Interactions
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Dahm, G. M., Gubin, M. M., Magee, J. D., Techasintana, P., Calaluce, R., Atasoy, U. Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip. J. Vis. Exp. (67), e3851, doi:10.3791/3851 (2012).
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