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Primary Microglia Isolation from Mixed Glial Cell Cultures of Neonatal Rat Brain Tissue
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Primary Microglia Isolation from Mixed Glial Cell Cultures of Neonatal Rat Brain Tissue
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10:20 min
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August 15, 2012
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Isolating primary microglia from the cellular heterogeneity of the brain is essential to investigate their role in both physiological and pathological conditions. This protocol describes a mechanical isolation and mixed cell culture technique that provides high yield and high purity, viable primary microglial cells for in vitro study and downstream applications.
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Tamashiro, T. T., Dalgard, C. L., Byrnes, K. R. Primary Microglia Isolation from Mixed Glial Cell Cultures of Neonatal Rat Brain Tissue. J. Vis. Exp. (66), e3814, doi:10.3791/3814 (2012).
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