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使用具体远藤和exoglycosidases糖基化蛋白质的鉴定和表征
JoVE Journal
Biyoloji
This content is Free Access.
JoVE Journal Biyoloji
Identification and Characterization of Protein Glycosylation using Specific Endo- and Exoglycosidases

使用具体远藤和exoglycosidases糖基化蛋白质的鉴定和表征

DOI:

09:54 min

December 26, 2011

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Bölümler

  • 00:05Başlık
  • 01:46Enzymatic Deglycosylation
  • 03:32SDS-PAGE of Deglycosylated Samples
  • 04:55Pro-Q Emerald 300 for Detection of Glyclosylated Proteins in SDS-PAGE Gels
  • 06:49Analysis of hCGβ Enzymatic Deglycosylation
  • 09:02Conclusion

Özet

Otomatik Çeviri

使用特定的糖苷酶,以消除通过SDS - PAGE的糖蛋白的糖是一种有价值的的方法来检测蛋白质样品聚糖修改初始糖生物学研究的是一个不错的选择。以下deglycosylation变化可以检测凝胶的流动性的变化或与多糖敏感的试剂染色。

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