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Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique
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Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique

Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique

39,354 Views

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07:18 min

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October 27, 2011

DOI:

10.3791/3255-v

DOI:
10.3791/3255-v

•

07:18 min
October 27, 2011

•

14 Views
,
1Department of Molecular Oncology, Weatherall Institute of Molecular Medicine,University of Oxford , 2Institute of Genetics and Biotechnology, Faculty of Biology,University of Warsaw

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DT40, a model vertebrate genetic system, provides a powerful tool to analyze protein function. Here we describe a simple method that allows qualitative analysis of parameters that influence DNA synthesis during the S-phase in DT40 cells at the single molecule level.

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Schwab, R. A., Niedzwiedz, W. Visualization of DNA Replication in the Vertebrate Model System DT40 using the DNA Fiber Technique. J. Vis. Exp. (56), e3255, doi:10.3791/3255 (2011).

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