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Video-rate Scanning Confocal Microscopy and Microendoscopy
JoVE Journal
Biyomühendislik
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JoVE Journal Biyomühendislik
Video-rate Scanning Confocal Microscopy and Microendoscopy

Video-rate Scanning Confocal Microscopy and Microendoscopy

DOI:
10.3791/3252-v

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14:10 min

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October 20, 2011

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,
1Program in Biophysics,Harvard University , 2Division of Health Sciences and Technology,Harvard-MIT, 3Wellman Center for Photomedicine, Massachusetts General Hospital,Harvard Tıp Fakültesi

Bölümler

  • 00:05Başlık
  • 01:01Setting Up the Resonant Galvanometric Mirror and Relay Optics
  • 04:56Setting Up the Second Scanning Mirror and Rotating the Microscope
  • 06:37Setting Up the Scan, Tube, and Objective Lenses
  • 08:17Setting Up and Aligning the Confocal Pinhole and Detector
  • 12:38The Assembled System and Representative Images
  • 13:43Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

The complete construction of a custom, real-time confocal scanning imaging system is described. This system, which can be readily used for video-rate microscopy and microendoscopy, allows for an array of imaging geometries and applications not accessible using standard commercial confocal systems, at a fraction of the cost.

Etiketler

Confocal MicroscopyHigh-resolution ImagingOptical SectioningConfocal MicroendoscopyThree-dimensional ResolutionFluorescenceWide-field MicroscopeConfocal ApertureScanning MirrorsPrimary Dichroic Mirror

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