Dergi
/
İmmünoloji ve Enfeksiyon
/
Live Cell Imaging of Bacillus subtilis and Streptococcus pneumoniae using Automated Time-lapse Microscopy
JoVE Journal
İmmünoloji ve Enfeksiyon
This content is Free Access.
JoVE Journal İmmünoloji ve Enfeksiyon
Live Cell Imaging of Bacillus subtilis and Streptococcus pneumoniae using Automated Time-lapse Microscopy

Live Cell Imaging of Bacillus subtilis and Streptococcus pneumoniae using Automated Time-lapse Microscopy

41,769 Views

•

07:31 min

•

July 28, 2011

DOI:

10.3791/3145-v

DOI:
10.3791/3145-v

•

07:31 min
July 28, 2011

•

5 Views
, , ,
1Molecular Genetics Group, Groningen Biomolecular Sciences and Biotechnology Institute, Centre for Synthetic Biology,Groningen Üniversitesi

Özet

Automatically generated

This protocol provides a step-by-step procedure to monitor single cell behavior of different bacteria in time using automated fluorescence time-lapse microscopy. Furthermore, we provide guidelines how to analyze the microscopy images.

Explore More Videos

Live Cell ImagingBacillus SubtilisStreptococcus PneumoniaeAutomated Time-lapse MicroscopySingle Cell StudiesMicroscopy MethodFluorescence MicroscopyGrowth And DivisionPhylogenetic Lineage TreesCell History And AncestryGene Expression DynamicsProtein Localization

Read Article

Cite this Article

de Jong, I. G., Beilharz, K., Kuipers, O. P., Veening, J. W. Live Cell Imaging of Bacillus subtilis and Streptococcus pneumoniae using Automated Time-lapse Microscopy. J. Vis. Exp. (53), e3145, doi:10.3791/3145 (2011).

Download .ris file

Copy

Share Video

.

Copy