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在酵母用于监测Mitophagy荧光显微镜检测酿酒酵母

JoVE Journal
Biyoloji

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JoVE Journal Biyoloji

A Fluorescence Microscopy Assay for Monitoring Mitophagy in the Yeast Saccharomyces cerevisiae

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在酵母用于监测Mitophagy荧光显微镜检测酿酒酵母

25,169 Views

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10:27 min

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July 18, 2011

DOI:

DOI:

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10:27 min

July 18, 2011

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4 Views

Dalibor Mijaljica¹, Mark Prescott¹, Rodney J. Devenish¹

¹Department of Biochemistry and Molecular Biology, School of Biomedical Sciences,Monash University

Özet

一个强大的方法来监视传递到细胞器的降解和回收的酵母液泡酸性流明描述。该方法依赖一种基因编码的双发射荧光pH值传感器，和可视化的单个细胞，用荧光显微镜对特定目标细胞器的标签。

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