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Ex Vivo Cultura de células humanas primarias epiteliales del tubo de Falopio
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May 09, 2011
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Özet
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La trompa de Falopio (FT) se está convirtiendo en un sitio alternativo de origen para el carcinoma de ovario seroso (SOC). Este protocolo describe un nuevo método para el aislamiento y<em> Ex vivo</em> Cultivo de células epiteliales del tubo de Falopio. Este recapitula el sistema<em> In vivo</emEpitelio> y permite el estudio de la patogénesis del SOC.
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Ex Vivo CulturePrimary Human Fallopian Tube Epithelial CellsEpithelial Ovarian CancerFemale Cancer MortalityUnited StatesBreast CarcinomaUterine CarcinomaOvarian Cancer Cure RatesScreening ToolsEarly Stage Disease DetectionSurgeryChemotherapyAdvanced Stage DiseaseDiffuse Abdominal InvolvementHeterogeneous DiseaseHistologic SubtypesSerous Ovarian Carcinoma (SOC)BRCA Genes MutationsExperimental ModelsCancer Cell LinesMouse ModelsInitiating Genetic EventsPathogenesis Of DiseaseFallopian Tube Origin Of SOCFallopian Tube Secretory Epithelial Cell (FTSEC)Cell Lines Culture Systems
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Fotheringham, S., Levanon, K., Drapkin, R. Ex Vivo Culture of Primary Human Fallopian Tube Epithelial Cells. J. Vis. Exp. (51), e2728, doi:10.3791/2728 (2011).
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