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Ex vivo-Kultur von primären humanen Eileiter Epithelzellen
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May 09, 2011
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Die Eileiter (FT) wird als Alternative vor Ort Ursprungsbezeichnungen für seröse Ovarialkarzinom (SOC) entstehen. Dieses Protokoll beschreibt eine neuartige Methode zur Isolierung und<em> Ex vivo</em> Kultur der Eileiter Epithelzellen. Dieses System rekapituliert die<em> In vivo</em> Epithel und ermöglicht das Studium der Pathogenese SOC.
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Ex Vivo CulturePrimary Human Fallopian Tube Epithelial CellsEpithelial Ovarian CancerFemale Cancer MortalityUnited StatesBreast CarcinomaUterine CarcinomaOvarian Cancer Cure RatesScreening ToolsEarly Stage Disease DetectionSurgeryChemotherapyAdvanced Stage DiseaseDiffuse Abdominal InvolvementHeterogeneous DiseaseHistologic SubtypesSerous Ovarian Carcinoma (SOC)BRCA Genes MutationsExperimental ModelsCancer Cell LinesMouse ModelsInitiating Genetic EventsPathogenesis Of DiseaseFallopian Tube Origin Of SOCFallopian Tube Secretory Epithelial Cell (FTSEC)Cell Lines Culture Systems
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Fotheringham, S., Levanon, K., Drapkin, R. Ex Vivo Culture of Primary Human Fallopian Tube Epithelial Cells. J. Vis. Exp. (51), e2728, doi:10.3791/2728 (2011).
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