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Methods Development for Blood Borne Macrophage Carriage of Nanoformulated Antiretroviral Drugs
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Methods Development for Blood Borne Macrophage Carriage of Nanoformulated Antiretroviral Drugs

Methods Development for Blood Borne Macrophage Carriage of Nanoformulated Antiretroviral Drugs

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18:46 min

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December 09, 2010

DOI:

10.3791/2460-v

DOI:
10.3791/2460-v

•

18:46 min
December 09, 2010

•

6 Views
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1Department of Pharmacology and Experimental Neuroscience,University of Nebraska Medical Center

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Nanoparticles of indinavir, ritonavir, efavirenz and atazanavir were manufactured using wet milling, homogenization and ultrasonication. These nanoformulations, collectively termed nanoformulated antiretroviral therapy (nanoART), assessed macrophage-based drug delivery. Monocyte-derived macrophage nanoART uptake, retention and sustained release were determined. These preliminary studies suggest the potential of nanoART for clinical use.

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Methods DevelopmentBlood BorneMacrophageNanoformulated Antiretroviral DrugsPharmacodynamicsBioavailabilityDrug ToxicitiesNanomedicineART RegimensComplianceDrug PharmacokineticsManufacturing NanoARTSurfactant Lipid CoatingWet MillingHigh-pressure HomogenizationPolymer SuspensionUltrasonicationPhysical PropertiesParticle SizeChargeShapeBiologic PropertiesCell UptakeRetentionCytotoxicityAntiretroviral EfficacyHuman Monocyte-derived Macrophages (MDM)Peripheral Blood Monocytes IsolationLeukopacks Purification

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Balkundi, S., Nowacek, A. S., Roy, U., Martinez-Skinner, A., McMillan, J., Gendelman, H. E. Methods Development for Blood Borne Macrophage Carriage of Nanoformulated Antiretroviral Drugs. J. Vis. Exp. (46), e2460, doi:10.3791/2460 (2010).

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