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Negatif Seçilebilir Marker Geliştirme Entamoeba histolytica</em
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December 12, 2010
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Biz olumsuz bir seçim sisteminin gelişme raporu<em> E. histolytica</em> Transgenik kimerik protein ekspresyonu (FCU1) ve prodrogu 5-florositozin seçimi dayalı. FCU1 protein, maya sitozin deaminaz ve phosphoribosyltransferase urasil bir füzyon. FCU1, İfade sonuçlandı<em> E. histolytica</em> 5-florositozin karşı duyarlılık.
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Negative Selectable MarkerEntamoeba HistolyticaAmebiasisGene ExpressionTransfectionPlasmidsReverse GeneticsHomologous RecombinationEpisomal Plasmid DNABackground Of Exogenous DNAGenomic Integration EventNegative Selection SystemYeast Cytosine Deaminase And Uracil Phosphoribosyl Transferase Chimera (FCU1)Prodrug 5-fluorocytosine (5-FC)5-fluorouracil5-fluorouridine-5′-monophosphate
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Abhyankar, M. M., Haviland, S. M., Gilchrist, C. A., Petri, Jr., W. A. Development of a Negative Selectable Marker for Entamoeba histolytica. J. Vis. Exp. (46), e2410, doi:10.3791/2410 (2010).
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