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等位基因特异性基因表达检测,测试的遗传协会的功能基础
JoVE Journal
Biyoloji
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JoVE Journal Biyoloji
An Allele-specific Gene Expression Assay to Test the Functional Basis of Genetic Associations

等位基因特异性基因表达检测,测试的遗传协会的功能基础

DOI:

10:17 min

November 03, 2010

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Bölümler

  • 00:00Başlık
  • 01:00Introduction
  • 01:45Cell Line Selection and Cell Culture
  • 03:10PCR and Primer Extension Assay
  • 05:50Experimental Controls
  • 09:30Conclusion

Özet

Otomatik Çeviri

遗传协会经常保持在功能级别上的原因不明。这种方法的目的是,通过分析细胞转录单核苷酸多态性杂合子基因表达,以评估的表型相关的遗传标记的影响。该技术可以精确测量,用MALDI - TOF质谱定量等位基因特异性引物延伸产品。

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