Blue Native Polyacrylamide Gel Electrophoresis (BN-PAGE) for Analysis of Multiprotein Complexes from Cellular Lysates

Gina J. Fiala; Wolfgang W. A. Schamel; Britta Blumenthal

doi:10.3791/2164

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Blauwe Inheemse polyacrylamidegelelektroforese (BN-PAGE) voor de Analyse van multiprotein complexen van Cellular Lysaten

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Blue Native Polyacrylamide Gel Electrophoresis (BN-PAGE) for Analysis of Multiprotein Complexes from Cellular Lysates

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Blauwe Inheemse polyacrylamidegelelektroforese (BN-PAGE) voor de Analyse van multiprotein complexen van Cellular Lysaten

DOI:

10.3791/2164-v

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12:03 min

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February 24, 2011

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Gina J. Fiala^2,3, Wolfgang W. A. Schamel*^2,3, Britta Blumenthal*³

¹Spemann Graduate School of Biology and Medicine (SGBM),University of Freiburg, ²Centre for Biological Signalling Studies (bioss) and Biology III, Faculty of Biology,University of Freiburg, ³Department of Molecular Immunology,Max-Planck-Institute of Immunology and Epigenetics

Bölümler

00:05Başlık
01:21Introduction
01:58Preparation of Dialyzed Cell Lysate
04:48Pouring of Blue Native-gels
07:04Separation of Dialyzed Cell Lysate by Blue Native-PAGE
07:53Second Dimension SDS-PAGE
09:25Representative Results for Blue Native-PAGE Analysis of Multiprotein Complexes from Cellular Lysates
11:18Conclusion

Özet

Otomatik Çeviri

In deze video, beschrijven we de karakterisering van multiprotein complexen (MPL), door blauwe inheemse polyacrylamide gel elektroforese (BN-PAGE). In een eerste dimensie, worden gedialyseerd cellulaire lysaten gescheiden door BN-PAGE om individuele Mediterrane partnerlanden te identificeren. In een tweede dimensie SDS-PAGE, zijn mediterrane partnerlanden van belang verder onderverdeeld naar hun kiezers te analyseren door immunoblotting.