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蓝原生聚丙烯酰胺凝胶电泳（BN - PAGE）分析，从细胞裂解液多蛋白复合物
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JoVE Journal Biyoloji
Blue Native Polyacrylamide Gel Electrophoresis (BN-PAGE) for Analysis of Multiprotein Complexes from Cellular Lysates
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蓝原生聚丙烯酰胺凝胶电泳（BN - PAGE）分析，从细胞裂解液多蛋白复合物

DOI:
10.3791/2164-v

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12:03 min

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February 24, 2011

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, ,
1Spemann Graduate School of Biology and Medicine (SGBM),University of Freiburg, 2Centre for Biological Signalling Studies (bioss) and Biology III, Faculty of Biology,University of Freiburg, 3Department of Molecular Immunology,Max-Planck-Institute of Immunology and Epigenetics

Bölümler

  • 00:05Başlık
  • 01:21Introduction
  • 01:58Preparation of Dialyzed Cell Lysate
  • 04:48Pouring of Blue Native-gels
  • 07:04Separation of Dialyzed Cell Lysate by Blue Native-PAGE
  • 07:53Second Dimension SDS-PAGE
  • 09:25Representative Results for Blue Native-PAGE Analysis of Multiprotein Complexes from Cellular Lysates
  • 11:18Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

在这个视频中，我们描述的多蛋白复合物（储值卡）蓝色原生聚丙烯酰胺凝胶电泳（BN - PAGE）的表征。在第一个层面，透析细胞裂解液的BN - PAGE分离，以确定个人的多用途储值卡。在第二维SDS - PAGE电泳，利益的储值卡是进一步细分免疫印迹分析其成分。

Etiketler

Blue Native Polyacrylamide Gel ElectrophoresisBN-PAGEMultiprotein ComplexesCell SignallingProtein-protein Interaction NetworksNative ConditionsResolutionGel FiltrationSucrose Density UltracentrifugationMPC SizeCompositionRelative AbundanceHydrodynamic Size And ShapePolyacrylamide MatrixCellular LysatesLysate DialysisFirst Dimension BN-PAGESecond Dimension SDS-PAGE

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