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Antikörper Profiling von Luciferase Immunpräzipitation Systems (LIPS)
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Antibody Profiling by Luciferase Immunoprecipitation Systems (LIPS)
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Antikörper Profiling von Luciferase Immunpräzipitation Systems (LIPS)

26,507 Views

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12:19 min

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October 07, 2009

DOI:

10.3791/1549-v

DOI:
10.3791/1549-v

•

12:19 min
October 07, 2009

•

10 Views
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1Neurobiology and Pain Therapeutics Section,National Institute of Dental and Craniofacial Research, National Institutes of Health

Özet

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Die technischen Aspekte der Durchführung LIPS (Luciferase Immunpräzipitation Systems) beschrieben sind. Das Gesamtkonzept beinhaltet Ausdruck chimären Gene Antigene fusioniert an Renilla Luziferase (RUC) in Säugerzellen. Crude Ruc-Antigen-Extrakte werden dann aufbereitet und ohne Reinigung eingesetzt in Immunpräzipitationsassays, um Antikörper zu quantifizieren.

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Burbelo, P. D., Ching, K. H., Klimavicz, C. M., Iadarola, M. J. Antibody Profiling by Luciferase Immunoprecipitation Systems (LIPS). J. Vis. Exp. (32), e1549, doi:10.3791/1549 (2009).

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