Canalostomy for Local Drug Delivery into the Inner Ear of an Adult Mouse

Published: May 31, 2024

Abstract

Source: Guo, J. et al., Canalostomy as a Surgical Approach to Local Drug Delivery into the Inner Ears of Adult and Neonatal Mice. J. Vis. Exp. (2018)

This video showcases canalostomy, a procedure that allows local drug delivery into the inner ear of a mouse through the semicircular canal with minimal damage to hearing and vestibular function. This method enables the inoculation of viral vectors, pharmaceuticals, and small molecules into the mouse's inner ear.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Device Preparations To make the injection cannula (Figure 1A), connect polyimide tubing (inner diameter 114.3 µm, outer diameter 139.7 µm, length ~3 cm) to polyethylene tubing (inner diameter 280 µm, outer diameter 640 µm, length ~40 cm). Using superglue, seal the connection with at …

Representative Results

Figure 1: Device preparation. (A) The polyimide tubing (i) and the polyethylene tubing (ii) are sealed to make an injection cannula (iii). (B) The cannula is connected to a 10-µL micro-syringe with a 30 G needle and then installed on a pump. Arrow indicates the cannula tip. (C) Injection reagent and normal saline are separated by an air gap. <p class="jo…

Açıklamalar

The authors have nothing to disclose.

Materials

Polymide Tubing A-M Systems 823400
Polyethylene Tubing Scientific Commodities Inc. BB31695-PE/1
10μl Microsyringe Hamilton Company 80001
Xylazine HCL Sigma-Aldrich Co. Llc. X-1251
Operating Miroscope Carl Zeiss Optical LLC. Pico
Micro Forceps Dumont Dumostar 10576
Fast-green Dye Sigma-Aldrich Co. Llc. F7252
AAV8-GFP BioMiao Biological Technology Co. Ltd (Beijing, China) 20161101 Titer: 2×10e12 vg/mL
Streptomycin Sulfate Sigma-Aldrich Co. Llc. S9137
Microinjection Pump Stoelting Co. 789100S
Electric Pad Pet Fun 11072931136
1 cc Syringe Mishawa Medical Industries Ltd. (Shanghai, China) 2011-3151258
Ketamine HCL Gutian Pharmaceutical Co., Ltd. (Fujian, China) H35020148
Electric Animal Clipper Codos Electrical Appliances Co., Ltd. (Guangdong, China) CP-8000
Cotton Pellet Yatai Healthcare Ltd. (Henan, China) Yu-2008-1640081
Suture Shanghai Pudong Jinhuan Medical Products Co., Ltd. (Shanghai, China) Hu-2013-2650207
Eye Ointment Beijing Shuangji Pharmaceutical Ltd. (Beijng China) H11021270

Etiketler

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Bu Makaleden Alıntı Yapın
Canalostomy for Local Drug Delivery into the Inner Ear of an Adult Mouse. J. Vis. Exp. (Pending Publication), e22275, doi: (2024).

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