Generation of a Mouse Model of Experimental Cerebral Malaria Using Host Mosquito-Derived Sporozoites
Published: October 31, 2023
Abstract
Source: Hoffmann, A. et al., In Vivo Tracking of Edema Development and Microvascular Pathology in a Model of Experimental Cerebral Malaria Using Magnetic Resonance Imaging. J. Vis. Exp. (2017)
This video demonstrates the generation of an experimental mouse model of cerebral malaria. Sporozoites isolated from Plasmodium berghei-infected female mosquitoes are injected into a restrained mouse's tail veins, leading to blood-brain barrier disruption and brain inflammation, causing cerebral malaria. This infected mouse model offers a valuable tool to study in vivo microvascular pathology associated with cerebral malaria.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Infection Infect Anopheles stephensi mosquitoes with Plasmodium berghei ANKA by feeding them for 15 min on a gametocytemic mouse. Keep the infected mosquitoes at 80% humidity and 21 °C. Collect female mosquitoes from their cage 17 to 22 days after the blood meal. Place them on ice to ane…
Açıklamalar
The authors have nothing to disclose.
Materials
| Isoflurane | Baxter | 1001747 | for anesthesia |
| Dotarem | Guebert | 1086923 | Gd-DTPA contrast agent; 0.5mmol/ml |
| Amira (Image Processing Program) | FEI Group | Version Amira 5.3.2 | |
| MATLAB | The MathWorks, Inc., | Release 2012b | |
| FDT toolbox | FMRIB's Software Library |
Etiketler
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Generation of a Mouse Model of Experimental Cerebral Malaria Using Host Mosquito-Derived Sporozoites. J. Vis. Exp. (Pending Publication), e21721, doi: (2023).