Toluidine Blue Staining of Peripheral Nerve Sections: A Technique to Stain Myelinated Nerve Fibers For Peripheral Nerve Structure Visualization
Published: April 30, 2023
Abstract
Source: Ghnenis, A. B. et al., Toluidine Blue Staining of Resin-Embedded Sections for Evaluation of Peripheral Nerve Morphology. J. Vis. Exp. (2018)
This video demonstrates the staining technique of resin-embedded, osmium tetroxide-treated rat peripheral nerve sections using toluidine blue. The stained nerve sections help visualize the fine structures of peripheral nerves.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Toluidine Blue Staining Prepare 1% solution of toluidine blue by dissolving 2 g of sodium borate in 100 mL of deionized water, then add 1 g of toluidine blue and stir until dissolved. Filter the solution using filter paper (pore size: 11 µm) and keep the solution in an opaque bottle at room temperature for up to tw…
Açıklamalar
The authors have nothing to disclose.
Materials
| Toluidine Blue O | Sigma-Aldrich | T3260 | |
| Sodium Tetraborate Decahydrate | Acros Organics | 205950010 | |
| Microscope Cover Glass | Fisher Scientific | 12545102 | |
| VWR Micro Slides, Superfrost Plus | VWR | 48311-703 | |
| 100 Watt Oven | Millipore | 6350115 | |
| Whatman Filter Paper | Sigma-Aldrich | WHA10010155 | |
| Olympus fluorescence microscope | Dual CCD Color and Monochrome Camera, DP80 | ||
| 3 mL plastic pipette | Sigma-Aldrich | Z331740 |
Etiketler
Bu Makaleden Alıntı Yapın
Toluidine Blue Staining of Peripheral Nerve Sections: A Technique to Stain Myelinated Nerve Fibers For Peripheral Nerve Structure Visualization. J. Vis. Exp. (Pending Publication), e21040, doi: (2023).