Nephrectomy in a Porcine Model: A Surgical Procedure to Retrieve Porcine Kidney Graft for Transplantation
Published: April 30, 2023
Abstract
Source: Liu, W. J. et al., Orthotopic Kidney Auto-Transplantation in a Porcine Model Using 24 Hours Organ Preservation and Continuous Telemetry. J. Vis. Exp. (2020)
This video demonstrates nephrectomy, the surgical procedure to remove one of the kidneys along with the section of the ureter. The blood vessels to and from the kidney are tied and divided. The excised kidney can be used for transplantation procedures.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Nephrectomy and kidney graft retrieval Following surgical disinfection and draping, perform a median laparotomy to open the abdomen (25-30 cm). Use a standard abdominal retractor to expose the surgical field. Use wet and warm abdominal towels to cover the colon and the small bowel. Ask the second assistant to hold the bowel to…
Açıklamalar
The authors have nothing to disclose.
Materials
| Gosset abdomenal wall spreader | CHIRU-INSTRUMENTE, Kaierstuhl,Germany | 09-621512 | Abdominal retractor |
| Prolene 2-0, blue monofil VISI-BLACK, FS needle | Johnson & Johnson Medical GmbH – Ethicon Deutschland, Norderstedt, Germany | EH7038H | Skin |
| Prolene 3-0,blue monofil,FS1 needle | Johnson & Johnson Medical GmbH – Ethicon Deutschland, Norderstedt, Germany | EH7694H | Skin |
| Prolene 5-0 (simply angulated, C1 needle) blue monofil VISI-BLACK | Johnson & Johnson Medical GmbH – Ethicon Deutschland, Norderstedt, Germany | EH7227H | Vascular |
| Prolene 5-0 (double armed, C1 needle) 60cm | Johnson & Johnson Medical GmbH – Ethicon Deutschland, Norderstedt, Germany | KBB5661H | Vascular |
| Buprenorphine (Buprenovet) | Bayer Vital AG, Leverkusen, Germany | 794-996 | Analgesia |
| Sempermed derma PF Surgical Gloves Seril Gr. 7, 7.5, 8 | Semperit investment Asia Pte Ltd, Singapore | 42,00,78,24,20,08,71,40,00,000 | Surgical gloves |
| Sentinex® PRO Surgical Gowns Spunlace XL 150cm | Lohmann & Rauscher GmbH & Co. KG, Neuwied, Germany | 19302 | Surgical gown |
| Telasorp Belly wipes (green 45x45cm) | PAUL HARTMANN AG,Heidenheim, Germany | 4542437 | Abdominal towel |
| DE BAKEY-SATINSKY vascular clamp 215mm | ASANUS Medizintechnik GmbH, Tuttlingen, Germany | GF1661 | Vascular clamp |
| Dissecting scissors Mayo,250 mm, 10" | ASANUS Medizintechnik GmbH, Tuttlingen, Germany | SC2232 | Scissors for dissection |
| Dissecting scissors Metzenbaum-Fino, 260 mm, 101/4" | ASANUS Medizintechnik GmbH, Tuttlingen, Germany | SC2290 | Scissors for dissection |
| Bipolar forceps 16 cm straight, Branch 0,30 mm pointed, universal fit | Bühler Instrumente Medizintechnik GmbH,Tuttlingen, Germany | 08/0016-A | Biopolar forceps |
| surgical scissors standard pointed-blunt (thread/cloth scissors)175 mm, 7" | ASANUS Medizintechnik GmbH, Tuttlingen, Germany | SC1522 | Surgical Scissors |
| Titanit vascular scissors POTTS-SMITH,185 mm, 71/4"60° | ASANUS Medizintechnik GmbH, Tuttlingen, Germany | SC8562 | Pott scissors |
| HF surgical device ICC 300, Electrocautery | Erbe Elektromedizin Gmbh; Tübingen, Germany | 20132-043 | Cautery, biopolar |
Etiketler
Bu Makaleden Alıntı Yapın
Nephrectomy in a Porcine Model: A Surgical Procedure to Retrieve Porcine Kidney Graft for Transplantation. J. Vis. Exp. (Pending Publication), e20912, doi: (2023).