Corneoscleral Button Isolation: A Surgical Procedure to Obtain Corneoscleral Button from Porcine Eye

Published: April 30, 2023

Abstract

Source: Okurowska, K., et al. Establishing a Porcine Ex Vivo Cornea Model for Studying Drug Treatments against Bacterial Keratitis. J. Vis. Exp. (2020).

In this video, we show a procedure to surgically isolate the corneoscleral button to generate an ex vivo porcine cornea model. The model is helpful for histological, pathological, and pharmacological studies due to its similarity to the human eye.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Sterilization CRITICAL STEP: Disinfect all forceps and scissors by soaking for 1 h in 5% (v/v) solution of Distel in distilled water, clean with a brush, rinse with tap water and sterilize in an oven at 185 °C for a minimum of 2 h. Sterilize all other glassware and reagents by autoclaving at 121 °C f…

Açıklamalar

The authors have nothing to disclose.

Materials

Cellstar 12 well plate Greiner Bio-One 665180
Distel Fisher Scientific 12899357
Forceps Fisher Scientific 15307805
PBS SLS P4417
Petri dish Fisher Scientific 12664785
Povidone iodine Weldricks pharmacy 2122828
Scalpel blade number 15 Fisher Scientific O305
Scalpel Swann Morton Fisher Scientific 11849002

Etiketler

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Bu Makaleden Alıntı Yapın
Corneoscleral Button Isolation: A Surgical Procedure to Obtain Corneoscleral Button from Porcine Eye. J. Vis. Exp. (Pending Publication), e20831, doi: (2023).

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