Transverse Aortic Arch Banding in Mouse Model: A Minimally Invasive Technique in Mice for Induction of High Pressure in the Left Ventricle
Published: April 30, 2023
Abstract
Source: Zaw, A. M. et al. Minimally Invasive Transverse Aortic Constriction in Mice. J. Vis. Exp. (2017)
In this video, we demonstrate an experimental protocol for minimally invasive transverse aortic constriction in mice to examine pressure overload-induced heart failure.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Operation Site Preparation Disinfect the operation site with 75% isopropyl alcohol. Adjust the heating pad temperature to 37 °C to avoid changes in heart and respiratory rates of the subject animal. Use surgical instruments sterilized in a gravity displacement sterilizer at 121 °C fo…
Representative Results
Figure 1: Process of forming the desired needle hooks by bending a 1-in, 30-gauge needle tip. (A) The original 1-in, 30-gauge needle tip. (B) Grasp the needle tip with the needle holder and bend approximately halfway along its length. Bend the needle into a loop, using the needle holder and the hands, as necessary, to form the loop pictured in th…
Açıklamalar
The authors have nothing to disclose.
Materials
| 1 inch 30-gauge Needles | BD, Franklin Lakes, NJ 07417, USA | 305128 | Curved as in the procedure and Figure 1 |
| 27-gauge Needle | BD, Franklin Lakes, NJ 07417, USA | 301629 | Make blunt and smooth for spacer |
| 6/0 Dafilon | B. Braun, 34212 Melsungen, Hessen, Germany | C0933066 | |
| 6/0 Silkam | B. Braun, 34212 Melsungen, Hessen, Germany | C0762067 | |
| Sterile gloves | A.R. Medicom, Inc (Asia), Hong Kong | ||
| Cotton Applicator | Mannings, Quarry Bay, Hong Kong | Local Shopping Center | |
| Depilatory Cream | Veet (Hong Kong), Kwun Tong, Kowloon, Hong Kong | Local Shopping Center | |
| Lexer-Baby Scissor | FST, British Columbia V7H 0A6, Canada | 14078-10 | |
| Curved Iris Forcep | FST, British Columbia V7H 0A6, Canada | 11065-07 | |
| Micro Olsen-Hegar Needle Holder | WPI, Sarasota, FL 34240, USA | 501989 | |
| Stereo Microscope | WPI, Sarasota, FL 34240, USA | PZMIII-BS | |
| GenTeal Eye Gel | Novartis, East Hanover, NJ 07936, USA | Local Pharmacy | |
| Heating Pad | Kent Scientific Corporation, Torrington, CT 06790, USA | DCT-20 | |
| Surgical tape | Laboratory Animal Unit | ||
| Ketamine and Xylazine | Laboratory Animal Unit | ||
| Betadine | Laboratory Animal Unit | ||
| Buprenorphine | Laboratory Animal Unit |
Etiketler
Bu Makaleden Alıntı Yapın
Transverse Aortic Arch Banding in Mouse Model: A Minimally Invasive Technique in Mice for Induction of High Pressure in the Left Ventricle. J. Vis. Exp. (Pending Publication), e20752, doi: (2023).