Murine Perineural Invasion Model: Generating an In Vivo Model to Simulate Perineural Invasion in Mouse Sciatic Nerve
Published: April 30, 2023
Abstract
Source: Deborde, S. et al. An In Vivo Murine Sciatic Nerve Model of Perineural Invasion. J. Vis. Exp. (2018)
In this video, we develop an in vivo mouse model for perineural invasion by injecting pancreatic cancer cells into the sciatic nerve. This model helps quantify the extent of invasion and study the interactions between cancer cells and nerves.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Preparation of the Mice and Surgery NOTE: 8-week-old, male and female C57BL/6J mice are used in this study. The surgery conditions follow the IACUC rules of our institution. The instruments are sterilized, the surgical working surface is disinfected, the animal is disinfected, and the surgeon wears sterile gloves. …
Açıklamalar
The authors have nothing to disclose.
Materials
| Mouse | Number and age variable depending on experimental needs | ||
| Electric razor | WAHL | 9962 | Can be substituted with commercial hair removal agent |
| Isoflurane, 250 mL | Baxter | 1001936060 | |
| Hypoallergenic surgical tape | 3M Blenderm | 70200419342 | |
| Betadine Swapsticks | PDI | SKU 41350 | |
| Webcol Alcohol Preps | Covidien | 5110 | |
| Sterile surgical tools (scissors and forceps) | |||
| 10 μL Hamilton syringe | Hamilton | 80308 | |
| Steel Micro spatula | Fisher Scientific | S50823 | |
| Dissecting microscope | |||
| Bupivacine, 1 g | Enzo Life Sciences | BML-NA139-0001 | Reconstitute to 0.5% |
| 5-0 Nylon suture | Ethicon | 698H |
Etiketler
Bu Makaleden Alıntı Yapın
Murine Perineural Invasion Model: Generating an In Vivo Model to Simulate Perineural Invasion in Mouse Sciatic Nerve. J. Vis. Exp. (Pending Publication), e20686, doi: (2023).