Preparation of Decellularized Rat Kidney: A Procedure to Generate Acellular Vascularized Whole-organ Scaffold from a Harvested Rat Kidney
Published: February 22, 2023
Abstract
Source: Kim, E. H. et al. Preparation of Decellularized Kidney Scaffolds in Rats. J. Vis. Exp. (2021)
This video describes the preparation of a decellularized rat kidney. The obtained acellular vascular scaffold can serve as a promising platform for tissue engineering of organ grafts.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Kidney Harvesting and Decellularization Harvest the left kidney with the abdominal aorta and inferior vena cava. Ligate the ureter, thoracic aorta, superior vena cava, and branches of the abdominal aorta. Keep the organ hydrated in Dulbecco's PBS (DPBS) in a 10 cm Petri dish. Cannulate …
Açıklamalar
The authors have nothing to disclose.
Materials
| Polyethelyne 50 tubing, catheter tubing 100 ft | Braintree Scientific | .023" × .038” | |
| 3-0 silk spool vascular access/ ligation in rat | Braintree Scientific | SUT-S 110 | |
| Fine scissors to cut fascia/ connective tissue | Fine Science Tools | 14058-09 | |
| 25 gauge inch guide needle(for vascular catheters) | Becton Dickinson | 305145 | |
| Schwartz microserrefine vascular clamps | Fine Science Tools | 18052-01 (straight) | |
| 5-0 silk spool vascular access/ ligation in mouse | Braintree Scientific | SUT-S 106 |
Etiketler
Bu Makaleden Alıntı Yapın
Preparation of Decellularized Rat Kidney: A Procedure to Generate Acellular Vascularized Whole-organ Scaffold from a Harvested Rat Kidney. J. Vis. Exp. (Pending Publication), e20552, doi: (2023).