Source: Nath, D. et al. Identification, Histological Characterization, and Dissection of Mouse Prostate Lobes for In Vitro 3D Spheroid Culture Models. J. Vis. Exp. (2018)
In this video, we describe the procedure for the dissection of the urogenital system or UGS from a male mouse model. This UGS is further dissected ex vivo to isolate the prostate gland and study its role in prostate cancer development.
All procedures involving animals have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.
1. The Urogenital System (UGS) Dissection
NOTE: The schematic is presented in Figure 1.
Figure 1: Schematic flowchart demonstrating dissection of the mouse urogenital system and isolation of the prostate. Flow chart for the protocol steps described in sections 1
Figure 2: Dissection of the mouse UGS. Step-by-step images from the dissection of the UGS from a 3-month-old mouse: (a and b) making the incisions, (c-e) pulling back the skin to expose the abdominal area, (f) shifting the intestines, (g) moving the abdominal fat pads, (h and i) extracting the UGS from the abdominal cavity, (j) the extracted UGS, (k) the extracted UGS with the different organs and tissues marked as follows: SV (yellow) = seminal vesicles; P (red) = prostate; F (purple) = fat; V (light blue) = vas deferens; and B (green) = bladder.
The authors have nothing to disclose.
Mouse surgical instruments (Mouse Dissecting kit) |
World Precision Instruments | MOUSEKIT | |
Dissection microscope | |||
Dissection medium (DMEM + 10%FBS) |
Thermofisher Scientific | 11965-084 | |
Fetal Bovine Serum | Thermofisher Scientific | 10438018 |