Modeling Ovarian Cancer Cell Colonization of Omentum: An Ex Vivo Method to Establish Milky Spot Colonization of Fluorescently Labeled Ovarian Cancer Cells in Murine Omentum Explants
Published: April 30, 2023
Abstract
Source: Krishnan, V. et al. In Vivo and Ex Vivo Approaches to Study Ovarian Cancer Metastatic Colonization of Milky Spot Structures in Peritoneal Adipose. J. Vis. Exp. (2015)
In this video, we establish an ex vivo omental organ culture overlaid with GFP-expressing ovarian cancer cells to study ovarian cancer colonization within the omental explant. This protocol analyzes immune cell structures within the omentum known as milky spots that promote metastases.
Protocol
All procedures involving animals have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Milky Spot Colonization Ex Vivo Establishment of basic ex vivo omental organ culture conditions Place each omentum into a single culture plate insert and place within one well of a 12-well tissue culture plate containing 500 µl of DME/F12 media containing 20% FCS. Maintain organ cultures at 37 °C in a 5%…
Açıklamalar
The authors have nothing to disclose.
Materials
| Dissection Tools | Fine Science Tools | NA | |
| Geimsa | Fluka/Sigma Aldrich | 48900 | |
| 5% Formalin | Sigma | HT501320 | |
| DMEM | Corning | 10-013-CV | |
| PBS | Corning | 21-040-CV | Without calcium and Magnesium |
| Collagenase | Worthington | LS004196 | |
| Millicell culture plate insert | Millipore | PICM01250 | |
| Cell-Tak | Corning | 354240 |
Etiketler
Bu Makaleden Alıntı Yapın
Modeling Ovarian Cancer Cell Colonization of Omentum: An Ex Vivo Method to Establish Milky Spot Colonization of Fluorescently Labeled Ovarian Cancer Cells in Murine Omentum Explants. J. Vis. Exp. (Pending Publication), e20379, doi: (2023).